Like airport security barriers that either clear authorized travelers or block unauthorized ones from accessing central operation areas, the blood-brain barrier (BBB) tightly controls the transport of essential nutrients and energy metabolites into the brain and staves off unwanted substances circulating in the bloodstream. Its highly organized structure of thin blood vessels and supporting cells is also the major obstacle preventing lifesaving drugs from reaching the brain to effectively treat cancer, neurodegeneration, and other diseases of the central nervous system. In a number of brain diseases, the BBB can also break down locally, causing neurotoxic substances, blood cells, and pathogens to leak into the brain and wreak irreparable havoc.

To study the BBB and drug transport across it, researchers have mostly relied on animal models such as mice. However, the precise makeup and transport functions of BBBs in these models can significantly differ from those in human patients, which makes them unreliable for predicting drug delivery and therapeutic efficacies. Also, in vitromodels attempting to re-create the human BBB using primarily brain tissue-derived cells thus far have not been able to mimic the BBB’s physical barrier, transport functions, and drug and antibody shuttling activities closely enough to be useful as therapeutic development tools.

Now, a team led by Donald Ingber, founding director of Harvard’s Wyss Institute for Biologically Inspired Engineering, has overcome these limitations by leveraging its microfluidic organs-on-chips technology in combination with a developmentally inspired hypoxia-mimicking approach to differentiate human pluripotent stem (iPS) cells into brain microvascular endothelial cells (BMVECs). The resulting “hypoxia-enhanced BBB chip” recapitulates the cellular organization, tight barrier functions, and transport abilities of the human BBB, while allowing transport of drugs and therapeutic antibodies in a way that more closely mimics transport across the BBB in vivo than existing in vitro systems do. Their study is reported in Nature Communications.

“Our approach to modeling drug and antibody shuttling across the human BBB in vitro with such high and unprecedented fidelity presents a significant advance over existing capabilities in this enormously challenging research area,” said Ingber, who is also the Judah Folkman Professor of Vascular Biology at Harvard Medical School and professor of bioengineering at the John A. Paulson School of Engineering and Applied Sciences. “It addresses a critical need in drug-development programs throughout the pharma and biotech world that we now aim to help overcome with a dedicated brain-targeting program at the Wyss Institute using our unique talent and resources.”

The BBB consists of thin capillaries formed by BMVECs, multifunctional cells known as pericytes that wrap themselves around the outside of the vessels, and star-shaped astrocytes, which are non-neuronal brain cells that also contact blood vessels. In the presence of pericytes and astrocytes, endothelial cells can generate the tightly sealed vessel-wall barrier typical of the human BBB.

Ingber’s team first differentiated human iPS cells into brain endothelial cells in a culture dish using a method that was developed by co-author Eric Shusta, a professor of chemical and biological engineering at University of Wisconsin-Madison, but with the added power of bioinspiration. “Because in the embryo the BBB forms under low-oxygen conditions [hypoxia], we differentiated iPS cells for an extended time in an atmosphere with only 5 percent instead of the normal 20 percent oxygen concentration,” said co-first author Tae-Eun Park. “As a result, the iPS cells initiated a developmental program very similar to that in the embryo, producing BMVECs that exhibited higher functionality than BMVECs generated in normal oxygen conditions.” Park is a former postdoctoral fellow on Ingber’s team and is now an assistant professor at Ulsan National Institute of Science and Technology in the Republic of Korea.

Building on a previous human BBB model, the researchers next transferred the hypoxia-induced human BMVECs into one of two parallel channels of a microfluidic organ-on-chip device that are divided by a porous membrane and continuously perfused with medium. The other channel was populated with a mixture of primary human brain pericytes and astrocytes. Following an additional day of hypoxia treatment, the human BBB chip could be stably maintained for at least 14 days at normal oxygen concentrations, which is far longer than in vitro human BBB models attempted in the past.