“DNA bricks,” on the other hand, can assemble much larger structures from a multitude of short modular strands. However, their assembly requires tightly controlled environmental conditions, can be spuriously initiated in the absence of a seed, and produces a significant proportion of incomplete structures that need to be purified away.
“The introduction of DNA origami has been the single most impactful advance in the DNA nanotechnology field over the last two decades. The crisscross polymerization approach that we developed in this study builds off this and other foundations to extend controlled DNA self-assembly to much larger length scales,” said Shih, co-leader of the Wyss’ Molecular Robotics Initiative, and professor at Harvard Medical School and DFCI. “We envision that crisscross polymerization will be broadly enabling for all-or-nothing formation of two- and three-dimensional microstructures with addressable nanoscale features, algorithmic self-assembly, and zero-background signal amplification in diagnostic applications that require extreme sensitivity.”
Planting a seed
Having experienced the limitations of DNA origami and DNA brick nanostructures, the team started by asking if it was possible to combine the absolute seed-dependence of DNA origami assembly with the boundless size of DNA brick constructions in a third type of DNA nanostructure that grows rapidly and consistently to a large size.
“We argued that all-or-nothing assembly of micron-scale DNA structures could be achieved by designing a system that has a high free-energy barrier to spontaneous assembly. The barrier can only be bypassed with a seed that binds and arranges a set of ‘nucleating’ slats for joint capture of ‘growth’ slats. This initiates a chain reaction of growth-slat additions that results in long DNA ribbons,” said co-first author Dionis Minev, a postdoctoral fellow on Shih’s team.
“This type of highly cooperative, strictly seed-dependent nucleation follows some of the same principles governing cytoskeletal actin or microtubule filament initiation and growth in cells.”
The elongation of cytoskeletal filaments follows strict rules where each incoming monomer binds to several monomers that have previously been incorporated into the polymeric filament and in turn is needed for binding of the next one. “Crisscross polymerization takes this strategy to the next level by enabling non-nearest neighbors to be required for recruitment of incoming monomers. The resulting extreme level of coordination is the secret sauce,” said Minev.
From concept to actual structure(s)
Putting their concept into practice, the team designed and validated a system in which a tiny seed structure offers a high starting concentration of pre-formed binding sites in the form of protruding single DNA strands. These can be detected by DNA slats with six — or eight in an alternative crisscross system — available binding sites, each binding to one of six (or eight) neighboring protruding ssDNA strands in a crisscross pattern, and subsequent DNA slats are then continuously added to the elongating structure.
“Our design is remarkable because we achieved fast growth of huge DNA structures, yet with nucleation control that is orders-of-magnitude greater than other approaches. It’s like having your cake and eating it too, because we readily created large-scale assemblies and did so only where and when we so desired,” said co-first author Chris Wintersinger, a Ph.D. student in Shih’s group who collaborated on the project with Minev. “The control we achieved with crisscross greatly exceeds that observed for existing DNA methods where nucleation can only be directed within a narrow window of conditions where growth is exceedingly slow.”
Using crisscross polymerization, Shih’s team generated DNA ribbons that self-assembled as a result of a single specific seeding event into structures that measured up to tens of micrometers in length, with a mass almost one hundred times larger than a typical DNA origami. Moreover, by leveraging the high programmability of slat conformations and interactions, the researchers created ribbons with distinct turns and twists, resulting in coiled and tube-like structures. In future studies, this could be leveraged to create functionalized structures that can benefit from spatially separated compartments.