Part of the process of creating sperm and egg cells, recombination occurs when the two copies of every gene a creature inherits — one from its mother, another from its father — are jumbled together, resulting in a unique mix of both.

“It might seem that useful genes are more likely to be transferred between species,” said Michael Miyagi, the other main graduate student author. “That’s true, but there are also more mundane structural issues with the genome that mean some regions are more likely to have genes go back and forth.”

And whether those genes flow back and forth, Edelman said, often depends on how much those different regions recombine.

“In low-recombination regions, we tend to see more resistance to gene flow than in high-recombination regions,” he said. “What we think happens is that in very-high-recombination regions, the genes that are resistant or incompatible become dissociated from the genes that can flow across the species boundary.”

And in a lucky break, Miyagi said, the team identified a key gene that acts to switch color patterns as one of those that moved between species.

“Heliconius are famous for their color patterns … and we found that in one particular region of the genome there are about 500,000 base pairs that have been inverted relative to the ancestral sequence,” he said. “And smack in the middle of that inversion is that gene that we know controls color pattern. When you have an inversion like that it means you’re keeping all the things within it together, so they can’t recombine.”

In another case, Edelman said, the team discovered an even larger inversion on a different chromosome that remains a complete mystery.

The team was able to show that one of those inverted sequences was transferred, Mallet and Edelman said, thanks to a new analysis method developed by Miyagi.

Ultimately, Mallet, Edelman, and Miyagi said, the study shows that hybridization — however unlikely it may be — is one way for species to derive their genomes and may be a key process in the creation of the diversity of life we see today.

“In nature, it’s very unlikely that any individual will mate with a member of another species,” Mallet said. “But over evolutionary time, it does happen.

“It probably only happens in the youngest groups of species — species that are rapidly evolving,” he continued. “Most of the diversity of life is probably created in these rapid radiations. They are involved in things like the origin of mammals. During these radiations, this could be an important means of shuffling variation and recombining adaptations from different lineages.”

This research was supported with funding from a SPARC grant from the Broad Institute of Harvard and MIT and startup and studentship funds from Harvard University.