Clues in clay

A team of applied physicists from Harvard’s School of Engineering and Applied Sciences (SEAS), Princeton, and Brandeis has demonstrated the formation of semipermeable vesicles from inorganic clay.

The research, published online this week in the journal Soft Matter, shows that clay vesicles provide an ideal container for the compartmentalization of complex organic molecules.

The authors say the discovery opens the possibility that primitive cells may have formed inside inorganic clay microcompartments.

“A lot of work, dating back several decades, explores the role of air bubbles in concentrating molecules and nanoparticles to allow interesting chemistry to occur,” says lead author Anand Bala Subramaniam, a doctoral candidate at SEAS.

The new research demonstrates that an air bubble covered in clay can easily transform into a water-filled clay vesicle, creating a safe compartment with pores that allow important molecules to enter but not leave.

“Clay-armored bubbles” form naturally when platelike particles of montmorillonite collect on the outer surface of air bubbles under water.

When the clay bubbles come into contact with simple organic liquids such as ethanol and methanol, which have a lower surface tension than water, the liquid wets the overlapping plates. As the inner surface of the clay shell becomes wet, the disturbed air bubble inside dissolves.

The resulting clay vesicle is a strong, spherical shell that creates a physical boundary between the water inside and the water outside. The translucent, cell-like vesicles are resilient enough to protect their contents in an aquatic environment such as the ocean.

Microscopic pores in the vesicle walls create a semi-permeable membrane that allows chemical building blocks to enter the “cell,” while preventing larger structures from leaving.

Scientists have studied montmorillonite, an abundant clay, for hundreds of years, and the mineral is known to serve as a chemical catalyst, encouraging lipids to form membranes and single nucleotides to join into strands of RNA.

Because liposomes and RNA would have been essential precursors to primordial life, Subramaniam and his co-authors suggest that the pores in the clay vesicles could do double duty as both selective entry points and catalytic sites.

“The conclusion here is that small fatty acid molecules go in and self-assemble into larger structures, and then they can’t come out,” says principal investigator Howard A. Stone, the Dixon Professor in Mechanical and Aerospace Engineering at Princeton, and a former Harvard faculty member. “If there is a benefit to being protected in a clay vesicle, this is a natural way to favor and select for molecules that can self-organize.”

Future research will explore the physical interactions between the platelike clay particles, and between the liquids and the clay. The researchers are also interested in seeing whether these clay vesicles can, indeed, be found in the natural environment today.

“Whether clay vesicles played a significant role in the origins of life is of course unknown,” says Subramaniam, “but the fact that they are so robust, along with the well-known catalytic properties of clay, suggests that they may have had some part to play.”

Subramaniam and Stone’s co-authors include Jiandi Wan of Princeton University and Arvind Gopinath of Brandeis University.

The research was funded by the Harvard Materials Research Science and Engineering Center and supported by the Harvard Center for Brain Science imaging facility.