{"id":135806,"date":"2013-04-17T15:17:21","date_gmt":"2013-04-17T19:17:21","guid":{"rendered":"\/gazette\/?p=135806"},"modified":"2013-04-17T15:17:21","modified_gmt":"2013-04-17T19:17:21","slug":"coelacanth-genome-surfaces","status":"publish","type":"post","link":"https:\/\/news.harvard.edu\/gazette\/story\/2013\/04\/coelacanth-genome-surfaces\/","title":{"rendered":"Coelacanth genome surfaces"},"content":{"rendered":"\n\t\n\t
\n\t\t\t\n\t\t\tHealth\t\t<\/a>\n\t\t\n\t\t

\n\t\tCoelacanth genome surfaces\t<\/h1>\n\n\t\n\t\n\t
\n\t\t
\n\t\t\t
\n\t\t\t\t\t

\n\t\tHaley Bridger\t<\/p>\n\t\t\t

\n\t\t\tBroad Institute Communications\t\t<\/p>\n\t\t\t\t\t<\/address>\n\t\t<\/div>\n\n\t\t

\n\t\t\tUnexpected insights from a fish with a 300-million-year-old fossil record\t\t<\/h2>\n\t\t\n<\/header>\n\n\n\n
\n\n\n\t\t

An international team of researchers has decoded the genome of a creature whose evolutionary history is both enigmatic and illuminating: the African coelacanth. A sea-cave dwelling, 5-foot-long fish with limblike fins, the coelacanth was once thought to be extinct. A living coelacanth was discovered off the African coast in 1938, and since then, questions about these ancient-looking fish have loomed large.<\/p>\n

Coelacanths today closely resemble the fossilized skeletons of their ancestors of more than 300 million years ago. Their genome confirms what many researchers had long suspected: Genes in coelacanths are evolving more slowly than in other organisms.<\/p>\n

\u201cWe found that the genes overall are evolving significantly slower than in every other fish and land vertebrate that we looked at,\u201d said Jessica Alf\u00f6ldi, a research scientist at the Broad Institute of Harvard and MIT<\/a> and co-first author of a paper on the coelacanth genome, which appears in Nature<\/a> this week. \u201cThis is the first time that we\u2019ve had a big enough gene set to really see that.\u201d<\/p>\n

Researchers hypothesize that this slow rate of evolution may be because coelacanths simply have not needed to change: They live primarily off the Eastern African coast (a second coelacanth species lives off the coast of Indonesia), at ocean depths where relatively little has changed over the millennia.<\/p>\n

\u201cWe often talk about how species have changed over time,\u201d said Kerstin Lindblad-Toh<\/a>, scientific director of the Broad Institute\u2019s vertebrate genome biology group and senior author. \u201cBut there are still a few places on earth where organisms don\u2019t have to change, and this is one of them. Coelacanths are likely very specialized to such a specific, non-changing, extreme environment \u2014 it is ideally suited to the deep sea just the way it is.\u201d<\/p>\n

Because of their resemblance to fossils dating back millions of years, coelacanths today are often referred to as \u201cliving fossils\u201d \u2014 a term coined by Charles Darwin. But the coelacanth is not a relic of the past brought back to life: It is a species that has survived, reproduced, but changed very little in appearance for millions of years. \u201cIt\u2019s not a living fossil; it\u2019s a living organism,\u201d said Alf\u00f6ldi. \u201cIt doesn\u2019t live in a time bubble; it lives in our world, which is why it\u2019s so fascinating to find out that its genes are evolving more slowly than ours.\u201d<\/p>\n

The coelacanth genome has also allowed scientists to test other long-debated questions. For example, coelacanths possess some features that look oddly similar to those seen only in animals that dwell on land, including \u201clobed\u201d fins, which resemble the limbs of four-legged land animals (known as tetrapods). Another odd-looking group of fish known as lungfish possesses lobed fins, too. It is probable that one of the ancestral lobed-finned fish species gave rise to the first four-legged amphibious creatures to climb out of the water and up onto land, but until now, researchers could not determine which of the two was the likelier candidate.<\/p>\n

In addition to sequencing the full genome \u2014 nearly 3 billion \u201cletters\u201d of DNA \u2014 from the coelacanth, the researchers also looked at RNA content from the coelacanth (both the African and Indonesian species) and from the lungfish. This information allowed them to compare genes in use in the brain, kidneys, liver, spleen, and gut of lungfish with gene sets from coelacanths and 20 other vertebrate species. Their results suggested that tetrapods are more closely related to lungfish than to the coelacanth.<\/p>\n

However, the coelacanth is still a critical organism to study to understand what is often called the water-to-land transition. The lungfish may be more closely related to land animals, but its genome remains inscrutable: At 100 billion letters in length, the lungfish genome is simply too unwieldy for scientists to sequence, assemble, and analyze. The coelacanth\u2019s more modest genome (comparable in length to our own) is yielding valuable clues about the genetic changes that may have allowed tetrapods to flourish on land.<\/p>\n

By looking at what genes were lost when vertebrates came on land as well as what regulatory elements \u2014 parts of the genome that govern where, when, and to what degree genes are active \u2014 were gained, the researchers made several unusual discoveries:<\/p>\n

Sense of smell. <\/b>The team found that many regulatory changes influenced genes involved in smell perception and detecting airborne odors. They hypothesize that as creatures moved from sea to land, they needed new means of detecting chemicals in the environment around them.<\/p>\n

Immunity. <\/b>The researchers found a significant number of immune-related regulatory changes when they compared the coelacanth genome to the genomes of land animals. They hypothesized that these changes may have been part of a response to new pathogens encountered on land.<\/p>\n

Evolutionary development. <\/b>Researchers found several key genetic regions that may have been \u201cevolutionarily recruited\u201d to form tetrapod innovations such as limbs, fingers and toes, and the mammalian placenta. One of these regions, known as HoxD, harbors a particular sequence that is shared across coelacanths and tetrapods. It is likely that this sequence from the coelacanth was co-opted by tetrapods to help form hands and feet.<\/p>\n

Urea cycle. <\/b>Fish get rid of nitrogen by excreting ammonia into the water, but humans and other land animals quickly convert ammonia into less-toxic urea using the urea cycle. Researchers found that the most important gene involved in this cycle has been modified in tetrapods.<\/p>\n

The coelacanth genome may hold other clues for researchers investigating the evolution of tetrapods. \u201cThis is just the beginning of many analyses on what the coelacanth can teach us about the emergence of land vertebrates, including humans, and, combined with modern empirical approaches, can lend insights into the mechanisms that have contributed to major evolutionary innovations,\u201d said Chris Amemiya, a member of the Benaroya Research Institute and co-first author of the Nature paper. Amemiya is also a professor at the University of Washington.<\/p>\n

Sequencing the full coelacanth genome was uniquely challenging for many reasons. Coelacanths are an endangered species, so samples available for research are almost nonexistent. This meant that each sample obtained was precious: Researchers would have \u201cone shot\u201d at sequencing the collected genetic material, according to Alf\u00f6ldi. But the difficulties of obtaining a sample and the challenges of sequencing it also knit the community together.<\/p>\n

Although the coelacanth genome offers some tantalizing answers, the research team anticipates that further study of the fish\u2019s immunity, respiration, physiology, and more will lead to deep insights into how some vertebrates adapted to life on land, while others remained creatures of the sea.<\/p>\n\n\n<\/div>\n\n\t\t","protected":false},"excerpt":{"rendered":"

An international team of researchers has decoded the genome of a creature whose evolutionary history is both enigmatic and illuminating: the African coelacanth<\/p>\n","protected":false},"author":105622744,"featured_media":135810,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"gz_ga_pageviews":18,"gz_ga_lastupdated":"2022-05-03 06:38","document_color_palette":null,"author":"Haley Bridger","affiliation":"Broad Institute 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class=\"wp-block-post-author__content\">\n\t\t\t\t\t<p class=\"author wp-block-post-author__name\">\n\t\tHaley Bridger\t<\/p>\n\t\t\t<p class=\"wp-block-post-author__byline\">\n\t\t\tBroad Institute Communications\t\t<\/p>\n\t\t\t\t\t<\/address>\n\t\t<\/div>\n\n\t\t<time class=\"article-header__date\" datetime=\"2013-04-17\">\n\t\t\tApril 17, 2013\t\t<\/time>\n\n\t\t<span class=\"article-header__reading-time\">\n\t\t\t6 min read\t\t<\/span>\n\t<\/div>\n\n\t\t\t<\/div>\n\t\t\n\t\t\t<h2 class=\"article-header__subheading wp-block-heading\">\n\t\t\tUnexpected insights from a fish with a 300-million-year-old fossil record\t\t<\/h2>\n\t\t\n<\/header>\n"},"2":{"blockName":"core\/group","attrs":{"templateLock":false,"metadata":{"name":"Article content"},"align":"wide","layout":{"type":"constrained","justifyContent":"center"},"tagName":"div","lock":[],"className":"","style":[],"backgroundColor":"","textColor":"","gradient":"","fontSize":"","fontFamily":"","borderColor":"","ariaLabel":"","anchor":""},"innerBlocks":[{"blockName":"core\/freeform","attrs":{"content":"","lock":[],"metadata":[]},"innerBlocks":[],"innerHTML":"\n\t\t<p>An international team of researchers has decoded the genome of a creature whose evolutionary history is both enigmatic and illuminating: the African coelacanth. A sea-cave dwelling, 5-foot-long fish with limblike fins, the coelacanth was once thought to be extinct. A living coelacanth was discovered off the African coast in 1938, and since then, questions about these ancient-looking fish have loomed large.<\/p>\n<p>Coelacanths today closely resemble the fossilized skeletons of their ancestors of more than 300 million years ago. Their genome confirms what many researchers had long suspected: Genes in coelacanths are evolving more slowly than in other organisms.<\/p>\n<p>\u201cWe found that the genes overall are evolving significantly slower than in every other fish and land vertebrate that we looked at,\u201d said Jessica Alf\u00f6ldi, a research scientist at the <a href=\"http:\/\/www.broadinstitute.org\/\">Broad Institute of Harvard and MIT<\/a> and co-first author of a paper on the coelacanth genome, which appears in <a href=\"http:\/\/www.nature.com\/\">Nature<\/a> this week. \u201cThis is the first time that we\u2019ve had a big enough gene set to really see that.\u201d<\/p>\n<p>Researchers hypothesize that this slow rate of evolution may be because coelacanths simply have not needed to change: They live primarily off the Eastern African coast (a second coelacanth species lives off the coast of Indonesia), at ocean depths where relatively little has changed over the millennia.<\/p>\n<p>\u201cWe often talk about how species have changed over time,\u201d said <a href=\"http:\/\/www.broadinstitute.org\/scientific-community\/science\/programs\/genome-sequencing-and-analysis\/kerstin-lindblad-toh\">Kerstin Lindblad-Toh<\/a>, scientific director of the Broad Institute\u2019s vertebrate genome biology group and senior author. \u201cBut there are still a few places on earth where organisms don\u2019t have to change, and this is one of them. Coelacanths are likely very specialized to such a specific, non-changing, extreme environment \u2014 it is ideally suited to the deep sea just the way it is.\u201d<\/p>\n<p>Because of their resemblance to fossils dating back millions of years, coelacanths today are often referred to as \u201cliving fossils\u201d \u2014 a term coined by Charles Darwin. But the coelacanth is not a relic of the past brought back to life: It is a species that has survived, reproduced, but changed very little in appearance for millions of years. \u201cIt\u2019s not a living fossil; it\u2019s a living organism,\u201d said Alf\u00f6ldi. \u201cIt doesn\u2019t live in a time bubble; it lives in our world, which is why it\u2019s so fascinating to find out that its genes are evolving more slowly than ours.\u201d<\/p>\n<p>The coelacanth genome has also allowed scientists to test other long-debated questions. For example, coelacanths possess some features that look oddly similar to those seen only in animals that dwell on land, including \u201clobed\u201d fins, which resemble the limbs of four-legged land animals (known as tetrapods). Another odd-looking group of fish known as lungfish possesses lobed fins, too. It is probable that one of the ancestral lobed-finned fish species gave rise to the first four-legged amphibious creatures to climb out of the water and up onto land, but until now, researchers could not determine which of the two was the likelier candidate.<\/p>\n<p>In addition to sequencing the full genome \u2014 nearly 3 billion \u201cletters\u201d of DNA \u2014 from the coelacanth, the researchers also looked at RNA content from the coelacanth (both the African and Indonesian species) and from the lungfish. This information allowed them to compare genes in use in the brain, kidneys, liver, spleen, and gut of lungfish with gene sets from coelacanths and 20 other vertebrate species. Their results suggested that tetrapods are more closely related to lungfish than to the coelacanth.<\/p>\n<p>However, the coelacanth is still a critical organism to study to understand what is often called the water-to-land transition. The lungfish may be more closely related to land animals, but its genome remains inscrutable: At 100 billion letters in length, the lungfish genome is simply too unwieldy for scientists to sequence, assemble, and analyze. The coelacanth\u2019s more modest genome (comparable in length to our own) is yielding valuable clues about the genetic changes that may have allowed tetrapods to flourish on land.<\/p>\n<p>By looking at what genes were lost when vertebrates came on land as well as what regulatory elements \u2014 parts of the genome that govern where, when, and to what degree genes are active \u2014 were gained, the researchers made several unusual discoveries:<\/p>\n<p><b>Sense of smell. <\/b>The team found that many regulatory changes influenced genes involved in smell perception and detecting airborne odors. They hypothesize that as creatures moved from sea to land, they needed new means of detecting chemicals in the environment around them.<\/p>\n<p><b>Immunity. <\/b>The researchers found a significant number of immune-related regulatory changes when they compared the coelacanth genome to the genomes of land animals. They hypothesized that these changes may have been part of a response to new pathogens encountered on land.<\/p>\n<p><b>Evolutionary development. <\/b>Researchers found several key genetic regions that may have been \u201cevolutionarily recruited\u201d to form tetrapod innovations such as limbs, fingers and toes, and the mammalian placenta. One of these regions, known as HoxD, harbors a particular sequence that is shared across coelacanths and tetrapods. It is likely that this sequence from the coelacanth was co-opted by tetrapods to help form hands and feet.<\/p>\n<p><b>Urea cycle. <\/b>Fish get rid of nitrogen by excreting ammonia into the water, but humans and other land animals quickly convert ammonia into less-toxic urea using the urea cycle. Researchers found that the most important gene involved in this cycle has been modified in tetrapods.<\/p>\n<p>The coelacanth genome may hold other clues for researchers investigating the evolution of tetrapods. \u201cThis is just the beginning of many analyses on what the coelacanth can teach us about the emergence of land vertebrates, including humans, and, combined with modern empirical approaches, can lend insights into the mechanisms that have contributed to major evolutionary innovations,\u201d said Chris Amemiya, a member of the Benaroya Research Institute and co-first author of the Nature paper. Amemiya is also a professor at the University of Washington.<\/p>\n<p>Sequencing the full coelacanth genome was uniquely challenging for many reasons. Coelacanths are an endangered species, so samples available for research are almost nonexistent. This meant that each sample obtained was precious: Researchers would have \u201cone shot\u201d at sequencing the collected genetic material, according to Alf\u00f6ldi. But the difficulties of obtaining a sample and the challenges of sequencing it also knit the community together.<\/p>\n<p>Although the coelacanth genome offers some tantalizing answers, the research team anticipates that further study of the fish\u2019s immunity, respiration, physiology, and more will lead to deep insights into how some vertebrates adapted to life on land, while others remained creatures of the sea.<\/p>\n","innerContent":["\n\t\t<p>An international team of researchers has decoded the genome of a creature whose evolutionary history is both enigmatic and illuminating: the African coelacanth. A sea-cave dwelling, 5-foot-long fish with limblike fins, the coelacanth was once thought to be extinct. A living coelacanth was discovered off the African coast in 1938, and since then, questions about these ancient-looking fish have loomed large.<\/p>\n<p>Coelacanths today closely resemble the fossilized skeletons of their ancestors of more than 300 million years ago. Their genome confirms what many researchers had long suspected: Genes in coelacanths are evolving more slowly than in other organisms.<\/p>\n<p>\u201cWe found that the genes overall are evolving significantly slower than in every other fish and land vertebrate that we looked at,\u201d said Jessica Alf\u00f6ldi, a research scientist at the <a href=\"http:\/\/www.broadinstitute.org\/\">Broad Institute of Harvard and MIT<\/a> and co-first author of a paper on the coelacanth genome, which appears in <a href=\"http:\/\/www.nature.com\/\">Nature<\/a> this week. \u201cThis is the first time that we\u2019ve had a big enough gene set to really see that.\u201d<\/p>\n<p>Researchers hypothesize that this slow rate of evolution may be because coelacanths simply have not needed to change: They live primarily off the Eastern African coast (a second coelacanth species lives off the coast of Indonesia), at ocean depths where relatively little has changed over the millennia.<\/p>\n<p>\u201cWe often talk about how species have changed over time,\u201d said <a href=\"http:\/\/www.broadinstitute.org\/scientific-community\/science\/programs\/genome-sequencing-and-analysis\/kerstin-lindblad-toh\">Kerstin Lindblad-Toh<\/a>, scientific director of the Broad Institute\u2019s vertebrate genome biology group and senior author. \u201cBut there are still a few places on earth where organisms don\u2019t have to change, and this is one of them. Coelacanths are likely very specialized to such a specific, non-changing, extreme environment \u2014 it is ideally suited to the deep sea just the way it is.\u201d<\/p>\n<p>Because of their resemblance to fossils dating back millions of years, coelacanths today are often referred to as \u201cliving fossils\u201d \u2014 a term coined by Charles Darwin. But the coelacanth is not a relic of the past brought back to life: It is a species that has survived, reproduced, but changed very little in appearance for millions of years. \u201cIt\u2019s not a living fossil; it\u2019s a living organism,\u201d said Alf\u00f6ldi. \u201cIt doesn\u2019t live in a time bubble; it lives in our world, which is why it\u2019s so fascinating to find out that its genes are evolving more slowly than ours.\u201d<\/p>\n<p>The coelacanth genome has also allowed scientists to test other long-debated questions. For example, coelacanths possess some features that look oddly similar to those seen only in animals that dwell on land, including \u201clobed\u201d fins, which resemble the limbs of four-legged land animals (known as tetrapods). Another odd-looking group of fish known as lungfish possesses lobed fins, too. It is probable that one of the ancestral lobed-finned fish species gave rise to the first four-legged amphibious creatures to climb out of the water and up onto land, but until now, researchers could not determine which of the two was the likelier candidate.<\/p>\n<p>In addition to sequencing the full genome \u2014 nearly 3 billion \u201cletters\u201d of DNA \u2014 from the coelacanth, the researchers also looked at RNA content from the coelacanth (both the African and Indonesian species) and from the lungfish. This information allowed them to compare genes in use in the brain, kidneys, liver, spleen, and gut of lungfish with gene sets from coelacanths and 20 other vertebrate species. Their results suggested that tetrapods are more closely related to lungfish than to the coelacanth.<\/p>\n<p>However, the coelacanth is still a critical organism to study to understand what is often called the water-to-land transition. The lungfish may be more closely related to land animals, but its genome remains inscrutable: At 100 billion letters in length, the lungfish genome is simply too unwieldy for scientists to sequence, assemble, and analyze. The coelacanth\u2019s more modest genome (comparable in length to our own) is yielding valuable clues about the genetic changes that may have allowed tetrapods to flourish on land.<\/p>\n<p>By looking at what genes were lost when vertebrates came on land as well as what regulatory elements \u2014 parts of the genome that govern where, when, and to what degree genes are active \u2014 were gained, the researchers made several unusual discoveries:<\/p>\n<p><b>Sense of smell. <\/b>The team found that many regulatory changes influenced genes involved in smell perception and detecting airborne odors. They hypothesize that as creatures moved from sea to land, they needed new means of detecting chemicals in the environment around them.<\/p>\n<p><b>Immunity. <\/b>The researchers found a significant number of immune-related regulatory changes when they compared the coelacanth genome to the genomes of land animals. They hypothesized that these changes may have been part of a response to new pathogens encountered on land.<\/p>\n<p><b>Evolutionary development. <\/b>Researchers found several key genetic regions that may have been \u201cevolutionarily recruited\u201d to form tetrapod innovations such as limbs, fingers and toes, and the mammalian placenta. One of these regions, known as HoxD, harbors a particular sequence that is shared across coelacanths and tetrapods. It is likely that this sequence from the coelacanth was co-opted by tetrapods to help form hands and feet.<\/p>\n<p><b>Urea cycle. <\/b>Fish get rid of nitrogen by excreting ammonia into the water, but humans and other land animals quickly convert ammonia into less-toxic urea using the urea cycle. Researchers found that the most important gene involved in this cycle has been modified in tetrapods.<\/p>\n<p>The coelacanth genome may hold other clues for researchers investigating the evolution of tetrapods. \u201cThis is just the beginning of many analyses on what the coelacanth can teach us about the emergence of land vertebrates, including humans, and, combined with modern empirical approaches, can lend insights into the mechanisms that have contributed to major evolutionary innovations,\u201d said Chris Amemiya, a member of the Benaroya Research Institute and co-first author of the Nature paper. Amemiya is also a professor at the University of Washington.<\/p>\n<p>Sequencing the full coelacanth genome was uniquely challenging for many reasons. Coelacanths are an endangered species, so samples available for research are almost nonexistent. This meant that each sample obtained was precious: Researchers would have \u201cone shot\u201d at sequencing the collected genetic material, according to Alf\u00f6ldi. But the difficulties of obtaining a sample and the challenges of sequencing it also knit the community together.<\/p>\n<p>Although the coelacanth genome offers some tantalizing answers, the research team anticipates that further study of the fish\u2019s immunity, respiration, physiology, and more will lead to deep insights into how some vertebrates adapted to life on land, while others remained creatures of the sea.<\/p>\n"],"rendered":"\n\t\t<p>An international team of researchers has decoded the genome of a creature whose evolutionary history is both enigmatic and illuminating: the African coelacanth. A sea-cave dwelling, 5-foot-long fish with limblike fins, the coelacanth was once thought to be extinct. A living coelacanth was discovered off the African coast in 1938, and since then, questions about these ancient-looking fish have loomed large.<\/p>\n<p>Coelacanths today closely resemble the fossilized skeletons of their ancestors of more than 300 million years ago. Their genome confirms what many researchers had long suspected: Genes in coelacanths are evolving more slowly than in other organisms.<\/p>\n<p>\u201cWe found that the genes overall are evolving significantly slower than in every other fish and land vertebrate that we looked at,\u201d said Jessica Alf\u00f6ldi, a research scientist at the <a href=\"http:\/\/www.broadinstitute.org\/\">Broad Institute of Harvard and MIT<\/a> and co-first author of a paper on the coelacanth genome, which appears in <a href=\"http:\/\/www.nature.com\/\">Nature<\/a> this week. \u201cThis is the first time that we\u2019ve had a big enough gene set to really see that.\u201d<\/p>\n<p>Researchers hypothesize that this slow rate of evolution may be because coelacanths simply have not needed to change: They live primarily off the Eastern African coast (a second coelacanth species lives off the coast of Indonesia), at ocean depths where relatively little has changed over the millennia.<\/p>\n<p>\u201cWe often talk about how species have changed over time,\u201d said <a href=\"http:\/\/www.broadinstitute.org\/scientific-community\/science\/programs\/genome-sequencing-and-analysis\/kerstin-lindblad-toh\">Kerstin Lindblad-Toh<\/a>, scientific director of the Broad Institute\u2019s vertebrate genome biology group and senior author. \u201cBut there are still a few places on earth where organisms don\u2019t have to change, and this is one of them. Coelacanths are likely very specialized to such a specific, non-changing, extreme environment \u2014 it is ideally suited to the deep sea just the way it is.\u201d<\/p>\n<p>Because of their resemblance to fossils dating back millions of years, coelacanths today are often referred to as \u201cliving fossils\u201d \u2014 a term coined by Charles Darwin. But the coelacanth is not a relic of the past brought back to life: It is a species that has survived, reproduced, but changed very little in appearance for millions of years. \u201cIt\u2019s not a living fossil; it\u2019s a living organism,\u201d said Alf\u00f6ldi. \u201cIt doesn\u2019t live in a time bubble; it lives in our world, which is why it\u2019s so fascinating to find out that its genes are evolving more slowly than ours.\u201d<\/p>\n<p>The coelacanth genome has also allowed scientists to test other long-debated questions. For example, coelacanths possess some features that look oddly similar to those seen only in animals that dwell on land, including \u201clobed\u201d fins, which resemble the limbs of four-legged land animals (known as tetrapods). Another odd-looking group of fish known as lungfish possesses lobed fins, too. It is probable that one of the ancestral lobed-finned fish species gave rise to the first four-legged amphibious creatures to climb out of the water and up onto land, but until now, researchers could not determine which of the two was the likelier candidate.<\/p>\n<p>In addition to sequencing the full genome \u2014 nearly 3 billion \u201cletters\u201d of DNA \u2014 from the coelacanth, the researchers also looked at RNA content from the coelacanth (both the African and Indonesian species) and from the lungfish. This information allowed them to compare genes in use in the brain, kidneys, liver, spleen, and gut of lungfish with gene sets from coelacanths and 20 other vertebrate species. Their results suggested that tetrapods are more closely related to lungfish than to the coelacanth.<\/p>\n<p>However, the coelacanth is still a critical organism to study to understand what is often called the water-to-land transition. The lungfish may be more closely related to land animals, but its genome remains inscrutable: At 100 billion letters in length, the lungfish genome is simply too unwieldy for scientists to sequence, assemble, and analyze. The coelacanth\u2019s more modest genome (comparable in length to our own) is yielding valuable clues about the genetic changes that may have allowed tetrapods to flourish on land.<\/p>\n<p>By looking at what genes were lost when vertebrates came on land as well as what regulatory elements \u2014 parts of the genome that govern where, when, and to what degree genes are active \u2014 were gained, the researchers made several unusual discoveries:<\/p>\n<p><b>Sense of smell. <\/b>The team found that many regulatory changes influenced genes involved in smell perception and detecting airborne odors. They hypothesize that as creatures moved from sea to land, they needed new means of detecting chemicals in the environment around them.<\/p>\n<p><b>Immunity. <\/b>The researchers found a significant number of immune-related regulatory changes when they compared the coelacanth genome to the genomes of land animals. They hypothesized that these changes may have been part of a response to new pathogens encountered on land.<\/p>\n<p><b>Evolutionary development. <\/b>Researchers found several key genetic regions that may have been \u201cevolutionarily recruited\u201d to form tetrapod innovations such as limbs, fingers and toes, and the mammalian placenta. One of these regions, known as HoxD, harbors a particular sequence that is shared across coelacanths and tetrapods. It is likely that this sequence from the coelacanth was co-opted by tetrapods to help form hands and feet.<\/p>\n<p><b>Urea cycle. <\/b>Fish get rid of nitrogen by excreting ammonia into the water, but humans and other land animals quickly convert ammonia into less-toxic urea using the urea cycle. Researchers found that the most important gene involved in this cycle has been modified in tetrapods.<\/p>\n<p>The coelacanth genome may hold other clues for researchers investigating the evolution of tetrapods. \u201cThis is just the beginning of many analyses on what the coelacanth can teach us about the emergence of land vertebrates, including humans, and, combined with modern empirical approaches, can lend insights into the mechanisms that have contributed to major evolutionary innovations,\u201d said Chris Amemiya, a member of the Benaroya Research Institute and co-first author of the Nature paper. Amemiya is also a professor at the University of Washington.<\/p>\n<p>Sequencing the full coelacanth genome was uniquely challenging for many reasons. Coelacanths are an endangered species, so samples available for research are almost nonexistent. This meant that each sample obtained was precious: Researchers would have \u201cone shot\u201d at sequencing the collected genetic material, according to Alf\u00f6ldi. But the difficulties of obtaining a sample and the challenges of sequencing it also knit the community together.<\/p>\n<p>Although the coelacanth genome offers some tantalizing answers, the research team anticipates that further study of the fish\u2019s immunity, respiration, physiology, and more will lead to deep insights into how some vertebrates adapted to life on land, while others remained creatures of the sea.<\/p>\n"}],"innerHTML":"\n<div class=\"wp-block-group alignwide\">\n\n\n\n<\/div>\n","innerContent":["\n<div class=\"wp-block-group alignwide\">\n\n","\n\n<\/div>\n"],"rendered":"\n<div class=\"wp-block-group alignwide has-global-padding is-content-justification-center is-layout-constrained wp-block-group-is-layout-constrained\">\n\n\n\t\t<p>An international team of researchers has decoded the genome of a creature whose evolutionary history is both enigmatic and illuminating: the African coelacanth. A sea-cave dwelling, 5-foot-long fish with limblike fins, the coelacanth was once thought to be extinct. A living coelacanth was discovered off the African coast in 1938, and since then, questions about these ancient-looking fish have loomed large.<\/p>\n<p>Coelacanths today closely resemble the fossilized skeletons of their ancestors of more than 300 million years ago. Their genome confirms what many researchers had long suspected: Genes in coelacanths are evolving more slowly than in other organisms.<\/p>\n<p>\u201cWe found that the genes overall are evolving significantly slower than in every other fish and land vertebrate that we looked at,\u201d said Jessica Alf\u00f6ldi, a research scientist at the <a href=\"http:\/\/www.broadinstitute.org\/\">Broad Institute of Harvard and MIT<\/a> and co-first author of a paper on the coelacanth genome, which appears in <a href=\"http:\/\/www.nature.com\/\">Nature<\/a> this week. \u201cThis is the first time that we\u2019ve had a big enough gene set to really see that.\u201d<\/p>\n<p>Researchers hypothesize that this slow rate of evolution may be because coelacanths simply have not needed to change: They live primarily off the Eastern African coast (a second coelacanth species lives off the coast of Indonesia), at ocean depths where relatively little has changed over the millennia.<\/p>\n<p>\u201cWe often talk about how species have changed over time,\u201d said <a href=\"http:\/\/www.broadinstitute.org\/scientific-community\/science\/programs\/genome-sequencing-and-analysis\/kerstin-lindblad-toh\">Kerstin Lindblad-Toh<\/a>, scientific director of the Broad Institute\u2019s vertebrate genome biology group and senior author. \u201cBut there are still a few places on earth where organisms don\u2019t have to change, and this is one of them. Coelacanths are likely very specialized to such a specific, non-changing, extreme environment \u2014 it is ideally suited to the deep sea just the way it is.\u201d<\/p>\n<p>Because of their resemblance to fossils dating back millions of years, coelacanths today are often referred to as \u201cliving fossils\u201d \u2014 a term coined by Charles Darwin. But the coelacanth is not a relic of the past brought back to life: It is a species that has survived, reproduced, but changed very little in appearance for millions of years. \u201cIt\u2019s not a living fossil; it\u2019s a living organism,\u201d said Alf\u00f6ldi. \u201cIt doesn\u2019t live in a time bubble; it lives in our world, which is why it\u2019s so fascinating to find out that its genes are evolving more slowly than ours.\u201d<\/p>\n<p>The coelacanth genome has also allowed scientists to test other long-debated questions. For example, coelacanths possess some features that look oddly similar to those seen only in animals that dwell on land, including \u201clobed\u201d fins, which resemble the limbs of four-legged land animals (known as tetrapods). Another odd-looking group of fish known as lungfish possesses lobed fins, too. It is probable that one of the ancestral lobed-finned fish species gave rise to the first four-legged amphibious creatures to climb out of the water and up onto land, but until now, researchers could not determine which of the two was the likelier candidate.<\/p>\n<p>In addition to sequencing the full genome \u2014 nearly 3 billion \u201cletters\u201d of DNA \u2014 from the coelacanth, the researchers also looked at RNA content from the coelacanth (both the African and Indonesian species) and from the lungfish. This information allowed them to compare genes in use in the brain, kidneys, liver, spleen, and gut of lungfish with gene sets from coelacanths and 20 other vertebrate species. Their results suggested that tetrapods are more closely related to lungfish than to the coelacanth.<\/p>\n<p>However, the coelacanth is still a critical organism to study to understand what is often called the water-to-land transition. The lungfish may be more closely related to land animals, but its genome remains inscrutable: At 100 billion letters in length, the lungfish genome is simply too unwieldy for scientists to sequence, assemble, and analyze. The coelacanth\u2019s more modest genome (comparable in length to our own) is yielding valuable clues about the genetic changes that may have allowed tetrapods to flourish on land.<\/p>\n<p>By looking at what genes were lost when vertebrates came on land as well as what regulatory elements \u2014 parts of the genome that govern where, when, and to what degree genes are active \u2014 were gained, the researchers made several unusual discoveries:<\/p>\n<p><b>Sense of smell. <\/b>The team found that many regulatory changes influenced genes involved in smell perception and detecting airborne odors. They hypothesize that as creatures moved from sea to land, they needed new means of detecting chemicals in the environment around them.<\/p>\n<p><b>Immunity. <\/b>The researchers found a significant number of immune-related regulatory changes when they compared the coelacanth genome to the genomes of land animals. They hypothesized that these changes may have been part of a response to new pathogens encountered on land.<\/p>\n<p><b>Evolutionary development. <\/b>Researchers found several key genetic regions that may have been \u201cevolutionarily recruited\u201d to form tetrapod innovations such as limbs, fingers and toes, and the mammalian placenta. One of these regions, known as HoxD, harbors a particular sequence that is shared across coelacanths and tetrapods. It is likely that this sequence from the coelacanth was co-opted by tetrapods to help form hands and feet.<\/p>\n<p><b>Urea cycle. <\/b>Fish get rid of nitrogen by excreting ammonia into the water, but humans and other land animals quickly convert ammonia into less-toxic urea using the urea cycle. Researchers found that the most important gene involved in this cycle has been modified in tetrapods.<\/p>\n<p>The coelacanth genome may hold other clues for researchers investigating the evolution of tetrapods. \u201cThis is just the beginning of many analyses on what the coelacanth can teach us about the emergence of land vertebrates, including humans, and, combined with modern empirical approaches, can lend insights into the mechanisms that have contributed to major evolutionary innovations,\u201d said Chris Amemiya, a member of the Benaroya Research Institute and co-first author of the Nature paper. Amemiya is also a professor at the University of Washington.<\/p>\n<p>Sequencing the full coelacanth genome was uniquely challenging for many reasons. Coelacanths are an endangered species, so samples available for research are almost nonexistent. This meant that each sample obtained was precious: Researchers would have \u201cone shot\u201d at sequencing the collected genetic material, according to Alf\u00f6ldi. But the difficulties of obtaining a sample and the challenges of sequencing it also knit the community together.<\/p>\n<p>Although the coelacanth genome offers some tantalizing answers, the research team anticipates that further study of the fish\u2019s immunity, respiration, physiology, and more will lead to deep insights into how some vertebrates adapted to life on land, while others remained creatures of the sea.<\/p>\n\n\n<\/div>\n"}},"jetpack-related-posts":[{"id":148344,"url":"https:\/\/news.harvard.edu\/gazette\/story\/2013\/10\/macrofied\/","url_meta":{"origin":135806,"position":0},"title":"Macrofied","author":"harvardgazette","date":"October 22, 2013","format":false,"excerpt":"The close-up perspective of the macro lens turns everyday surfaces into dynamic landscapes.","rel":"","context":"In "Campus & Community"","block_context":{"text":"Campus & Community","link":"https:\/\/news.harvard.edu\/gazette\/section\/campus-community\/"},"img":{"alt_text":"","src":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2013\/10\/macrofied_02_605.jpg?resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2013\/10\/macrofied_02_605.jpg?resize=350%2C200 1x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2013\/10\/macrofied_02_605.jpg?resize=525%2C300 1.5x"},"classes":[]},{"id":160323,"url":"https:\/\/news.harvard.edu\/gazette\/story\/2014\/09\/linking-natural-mutants-and-evolution\/","url_meta":{"origin":135806,"position":1},"title":"Linking ‘natural mutants’ and evolution","author":"harvardgazette","date":"September 3, 2014","format":false,"excerpt":"Researchers uncovered a variety of features in the genomes of five species of African cichlid fish that enabled them to thrive in new habitats and ecological niches. The findings from these \u201cnatural mutants\u201d shed new light on the molecular process of evolution in all vertebrate species.","rel":"","context":"In "Health"","block_context":{"text":"Health","link":"https:\/\/news.harvard.edu\/gazette\/section\/health\/"},"img":{"alt_text":"","src":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2014\/09\/detail_of_sexually_dimorphic_mouth_structure_110941708_605.jpg?resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2014\/09\/detail_of_sexually_dimorphic_mouth_structure_110941708_605.jpg?resize=350%2C200 1x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2014\/09\/detail_of_sexually_dimorphic_mouth_structure_110941708_605.jpg?resize=525%2C300 1.5x"},"classes":[]},{"id":110542,"url":"https:\/\/news.harvard.edu\/gazette\/story\/2012\/05\/vivid-details\/","url_meta":{"origin":135806,"position":2},"title":"Vivid details","author":"harvardgazette","date":"May 17, 2012","format":false,"excerpt":"A landmark effort to sequence the genome of the butterfly Heliconius melpomene has revealed that it shares genes that control color patterns with two species that closely mimic its appearance \u2014 Heliconius timareta and Heliconius elevatus \u2014 suggesting that all three exchange genes as a result of occasional hybridization.","rel":"","context":"In "Health"","block_context":{"text":"Health","link":"https:\/\/news.harvard.edu\/gazette\/section\/health\/"},"img":{"alt_text":"","src":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2012\/05\/05-17-butterfly-605-b.jpg?resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2012\/05\/05-17-butterfly-605-b.jpg?resize=350%2C200 1x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2012\/05\/05-17-butterfly-605-b.jpg?resize=525%2C300 1.5x"},"classes":[]},{"id":151843,"url":"https:\/\/news.harvard.edu\/gazette\/story\/2014\/01\/neanderthals-dna-legacy-linked-to-modern-ailments\/","url_meta":{"origin":135806,"position":3},"title":"Neanderthals\u2019 DNA legacy linked to modern ailments","author":"harvardgazette","date":"January 29, 2014","format":false,"excerpt":"Remnants of Neanderthal DNA in modern humans are associated with genes affecting type 2 diabetes, Crohn\u2019s disease, lupus, biliary cirrhosis, and smoking behavior. They also concentrate in genes that influence skin and hair characteristics. At the same time, Neanderthal DNA is conspicuously low in regions of the X chromosome and\u2026","rel":"","context":"In "Health"","block_context":{"text":"Health","link":"https:\/\/news.harvard.edu\/gazette\/section\/health\/"},"img":{"alt_text":"","src":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2014\/01\/denisova_entrance_1.jpg?resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2014\/01\/denisova_entrance_1.jpg?resize=350%2C200 1x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2014\/01\/denisova_entrance_1.jpg?resize=525%2C300 1.5x"},"classes":[]},{"id":318944,"url":"https:\/\/news.harvard.edu\/gazette\/story\/2021\/01\/harvard-researchers-sequence-sapria-genome\/","url_meta":{"origin":135806,"position":4},"title":"\u2018The most charismatic and strange of all flowering plants\u2019","author":"Lian Parsons","date":"January 22, 2021","format":false,"excerpt":"Sapria genome shows astonishing gene loss and gene theft.","rel":"","context":"In "Science & Tech"","block_context":{"text":"Science & Tech","link":"https:\/\/news.harvard.edu\/gazette\/section\/science-technology\/"},"img":{"alt_text":"Sapria himalayana flower.","src":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2021\/01\/sapria4_2500.jpg?resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2021\/01\/sapria4_2500.jpg?resize=350%2C200 1x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2021\/01\/sapria4_2500.jpg?resize=525%2C300 1.5x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2021\/01\/sapria4_2500.jpg?resize=700%2C400 2x"},"classes":[]},{"id":304438,"url":"https:\/\/news.harvard.edu\/gazette\/story\/2020\/05\/a-connection-between-ancestry-and-the-molecular-makeup-of-cancer\/","url_meta":{"origin":135806,"position":5},"title":"Mapping the cancer connection","author":"harvardgazette","date":"May 11, 2020","format":false,"excerpt":"A new study takes the most comprehensive look to date at the connection between the ancestry and the molecular makeup of cancer.","rel":"","context":"In "Health"","block_context":{"text":"Health","link":"https:\/\/news.harvard.edu\/gazette\/section\/health\/"},"img":{"alt_text":"Illustration of cancer cells.","src":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2020\/05\/iStock-1014775514.jpg?resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/news.harvard.edu\/wp-content\/uploads\/2020\/05\/iStock-1014775514.jpg?resize=350%2C200 1x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2020\/05\/iStock-1014775514.jpg?resize=525%2C300 1.5x, https:\/\/news.harvard.edu\/wp-content\/uploads\/2020\/05\/iStock-1014775514.jpg?resize=700%2C400 2x"},"classes":[]}],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/posts\/135806","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/users\/105622744"}],"replies":[{"embeddable":true,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/comments?post=135806"}],"version-history":[{"count":0,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/posts\/135806\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/media\/135810"}],"wp:attachment":[{"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/media?parent=135806"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/categories?post=135806"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/tags?post=135806"},{"taxonomy":"format","embeddable":true,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/gazette-formats?post=135806"},{"taxonomy":"series","embeddable":true,"href":"https:\/\/news.harvard.edu\/gazette\/wp-json\/wp\/v2\/series?post=135806"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}